Hoenger Lab
University of Colorado at Boulder
Department of Molecular, Cellular and Developmental Biology

Structural and Functional Investigations
into Cytoskeletal Assemblies by
Cryo Electron Microscopy
and 3D Image Analysis

 
Resources:: Bio 3D lab
The Bio 3D Lab was founded by Keith Porter and has been in existence for over 25 years. Richard McIntosh followed Dr Porter as the Director, then in February of 2006 Andreas Hoenger took over as the current facility Director. The Bio 3D Lab stands on it's own as a research facility and the staff and their expertise acts as a resource for the research done in the Hoenger lab

The Journal of Cell Biology, volume 118

Eileen O'Toole, Boulder Bio 3D Lab

The Journal of Cell Biology, volume 144

Mark Ladinsky, Boulder Bio 3D Lab

Below: The trans side of the Golgi stack is at the top of the image, cis side is at the bottom.

The Boulder Laboratory for 3-Dimensional Electron Microscopy of Cells is located in the Department of Molecular, Cellular, and Developmental Biology at the University of Colorado. It is a National Resource Center supported by the National Center for Research Resources (NCRR) at the National Institutes of Health (NIH).

The Boulder Facility is devoted to the development of new technologies for specimen preparation and image analysis to study the three-dimensional structure of cells and tissues. Techniques for rapid-freezing and freeze-substitution of samples, immunolabeling, serial-sectioning and high-resolution electron tomography are employed and advanced in the facility.

The primary imaging tools of this facility are two FEI Tecnai intermediate voltage electron microscopes operating at 200,000 and 300,000 volts.

For more information about the Boulder 3D Laboratory and facilities please visit the web site http://bio3d.colorado.edu/

University of Colorado, MCDB, 347 UCB, Boulder, CO 80309-0347