Hoenger Lab
University of Colorado at Boulder
Department of Molecular, Cellular and Developmental Biology

Structural and Functional Investigations
into Cytoskeletal Assemblies by
Cryo Electron Microscopy
and 3D Image Analysis

 
Resources:: Electron Tomography
Electron tomography uses 2D images (electron tomographs) to derive a 3D model of an object. The process is accomplished by tilting the sample on the stage of the electron microscope and then taking a series of images that corresponds with each tilt angle. The image series are aligned and the 3D images are reconstructed from the alignments.
Electron tomograph
The begining of 3D modeling process
The images below illustrate the process of 3D reconstruction from a electron tomograph

For a description of the procedure and images and movies of tomographic data go to the Bio 3D Lab Home Page, then to the Movie and Datasets section, then to the Electron Tomography of Yeast Cells.

While on that page follow the other links to view some spectacular 3D reconstruction quicktime movies of Golgi ribbons, mitotic spindles, and kinetochores.
For more information about the Boulder 3D Laboratory and facilities please visit the web site http://bio3d.colorado.edu

University of Colorado, MCDB, 347 UCB, Boulder, CO 80309-0347