Hoenger Lab
University of Colorado at Boulder
Department of Molecular, Cellular and Developmental Biology

Selected Recent Publications:

Hoenger A, Nicastro D. Electron microscopy of microtubule-based cytoskeletal machinery. Methods Cell Biol. 2007;79:437-62. Review.

Norlen L, Masich S, Goldie KN, Hoenger A. Structural analysis of vimentin and keratin intermediate filaments by cryo-electron tomography. Exp Cell Res. 2007 Jun 10;313(10):2217-27.

Goldie, K.N., Wedig, T., Mitra, A., Aebi, U., Herrmann, H., and Hoenger, A. (2007) Dissecting the 3-D Structure of Vimentin Intermediate Filaments by Cryo-Electron Tomography. J. Struct. Biol. J Struct Biol. 2007 Jun;158(3):378-85.

Sandblad, L., Busch, E.K., Tittmann, P., Gross, H., Brunner, D., and Hoenger, A. (2006) The Schizosaccharomyces pombe EB1 Homolog Mal3p Binds and Stabilizes the Microtubule Lattice Seam. Cell, 127:1-10.

Santarella, R., Koffa, M., Tittmann, P., Gross, H., and Hoenger, A. (2006) HURP Wraps Microtubule-Ends With an Additional Tubulin Sheet That Has a Novel Conformation of Tubulin. J. Mol. Biol. Volume 365, Issue 5,2 February 2007, Pages 1587-1595

Iconomidou VA, Chryssikos GD, Gionis V, Galanis AS, Cordopatis P, Hoenger A, Hamodrakas SJ. (2006) Amyloid fibril formation propensity is inherent into the hexapeptide tandemly repeating sequence of the central domain of silkmoth chorion proteins of the A-family. 156:480-8.

Krzysiak, T.C., Wendt, T., Sproul, L.R., Gross, H., Gilbert, S.P. and Hoenger, A. (2006) A Structural Model for Monastrol Inhibition of Dimeric Kinesin Eg5. EMBO J. 2006 May 17; 25(10): 2263–2273.

Ribbeck K, Groen AC, Santarella R, Bohnsack MT, Raemaekers T, Kocher T, Gentzel M, Gorlich D, Wilm M, Carmeliet G, Mitchison TJ, Ellenberg J, Hoenger, A, Mattaj IW. NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules. Mol Biol Cell. 2006 Jun;17(6):2646-60

Hizlan D, Mishima M, Tittmann P, Gross H, Glotzer M, Hoenger A. (2006) Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubules. J Struct Biol. 153:73-84.

Marx A, Muller J, Mandelkow EM, Hoenger A, Mandelkow E. (2005) Interaction of kinesin motors, microtubules, and MAPs. J Muscle Res Cell Motil. 17:1-13.

Grabenbauer M, Geerts WJ, Fernadez-Rodriguez J, Hoenger A, Koster AJ, Nilsson T. (2005) Correlative microscopy and electron tomography of GFP through photooxidation. Nat Methods. 11:857-62.

Krebs, A., Goldie, K.N., and Hoenger, A. (2005) Structural rearrangements in alpha- and beta-tubulin upon microtubule formation. EMBO Reports, 6:227-32.

Santarella, R.A., Skiniotis, G., Goldie, K.N., Mandelkow, E-M., Gross, H., Mandelkow, E., and Hoenger, A. (2004) Microtubule Surface-Decoration Properties of Human TAU-40. J. Mol. Biol. 339: 539-553.

Schoehn, G., Mavrakis, M., Albertini, A., Wade, R., Hoenger, A., and Ruigrok, R.W.H. (2004) 12 Å Structure of Trypsin Treated Measles Virus N-RNA. J. Mol. Biol. 339: 301-312.

Klumpp, L.M., Hoenger, A., and Gilbert, S. (2004). Kinesin’s second step. Proc. Natl. Acad. Sci. 101: 3444-3449.

Skiniotis, G., Cochran, J. Gilbert, S.P., and Hoenger, A. (2004) The C-terminus of Tubulin Modulates Nucleotide-Dependent Kinesin Binding. EMBO J. 23: 989-999.

Hamodrakas, SJ., Hoenger, A., and Iconomidou, V.A. (2004) Amyloid fibrillogenesis of silkmoth chorion proteine peptide-analogues via a lipid-crystalline intermediate phase. J. Struct. Biol. 145:226-235.

Krebs, A., Goldie, K. N., and Hoenger, A. (2004) Complex Formation with Kinesin Motor Domains Affects the Structure of Microtubules. J. Mol. Biol. 335: 139-153.

Wendt, T., Karabay, A. Walker, R.A., Gross, H., and Hoenger, A. (2003) A Structural Analysis of the Interaction between Ncd Tail and Tubulin Protofilaments. J. Mol. Biol, 333: 541-552.

De Marco, V., Goldie, K.N., de Marco, A., Correia, J., and Hoenger, A. (2003) Dimerization Properties of a Xenopus Laevis Kinesin-II C-terminal Stalk Fragment. EMBO Reports, 4: 717-722.

Iconomidou, V.A., Chryssikos, G.D., Gionis, V., Hoenger, A., and Hamodrakas, S.J. (2003) FT-Raman spectroscopy as diagnostic tool of Congo red binding to amyloids. Biopolymers, 72: 185-92.

Schatz, C.A., Santarella, R., Hoenger, A., Karsenti, E., Mattaj, I.W., Gruss, O.J. and Carazo-Salas, R. E. (2003) Importin a-Regulated Nucleation of Microtubules by TPX2. EMBO J. 22: 2060-70.

Skiniotis, Y., Surrey, T., Altmann, S., Gross, H., Song Y.H., Mandelkow, E., and Hoenger, A. (2003) An SH3 Domain engineered between the Kinesin Linker and Neck allows a detailed Localization of the Neck-Linker Region by Cryo-Electron Microscopy. EMBO J. 22: 1518-28.

Raae, A.J., Bañuelos, S., Yiänne, J., Torbjörn, O., Goldie, K.N., Wendt, T., Hoenger, A., and Saraste, M. (2003) Actin binding of Minispectrin. BBA – Proteins and Proteomics, Vol. 1646: 67-76.

Klumpp, L.M., Brendza, K.M., Rosenberg, J.M., Hoenger, A., Gilbert, S.P. (2003) Motor domain Mutation traps Kinesin as a Microtubule-rigor Complex. Biochemistry, 42: 2595-2606.

Wendt, T.G., Volkmann, N., Goldie, K.N., Müller, J., Mandelkow, E., and Hoenger, A. (2002) Microtubule Binding Patterns of the Reverse Kinesin Motor Ncd reveal a Minus-End Directed Power Stroke. EMBO J. 21: 5969-5978.

López de la Paz, M., Goldie, K., Zurdo, J., Lacroix, E., Dobson, C.M., Hoenger, A., and Serrano, L. (2002) De novo designed peptide-based amyloid fibrils. Proc. Natl. Acad. Sci. 99: 16052-16057.

Resch, G. P., Goldie, K. N., Hoenger, A. and Small, J. V. (2002) Pure F-Actin Networks are Distorted and Branched by Steps in the Critical Point Drying Technique. J. Struct. Biol., 137, 305-312.

Resch, G. P., Goldie, K. N., Krebs, A., Hoenger, A. and Small, J. V. (2002) Visualisation of the Actin Cytoskeleton by Cryo-Electron Microscopy. J. Cell. Sci., 115, 1877-82.

Song, Y.H. Marx, A., Mueller, J., Woehlke, G., Schliwa, M., Krebs, A., Hoenger, A., and Mandelkow, E. (2001) Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules. EMBO J. 20: 6213-25.

De Marco, V., Burkhard, P., Le Bot, N., Vernos, I., and Hoenger, A. (2001) Analysis of heterodimer formation by Xklp3A/B, a newly cloned kinesin-II from Xenopus laevis. EMBO J. 20: 3370-9.

Iconomidou, V.A., Chryssikos, G.D., Gionis, V., Vriend, G., Hoenger, A., and Hamodrakas, S.J. (2001) Amyloid-like fibrils from an 18-residue peptide analogue of a part of the central domain of the B-family of silkmoth chorion proteins. FEBS Lett. 499: 268-73.

Beuron, F., and Hoenger, A. (2001) Structural analysis of the microtubule-kinesin complex by cryo-electron microscopy. Methods Mol. Biol. 164: 235-54.

Hoenger, A., Doerhoefer, M., Woehlke, G., Tittmann, P., Gross, H., Song, Y.-H., and Mandelkow, E. (2000) Surface Topography of Microtubule Walls Decorated with Monomeric and Dimeric Kinesin Constructs. Biol. Chem. 381: 1001-11.

Steinmetz, M.O., Hoenger, A., Stoffler, D., Noegel, A.A., Aebi, U., Schoenenberger, C.A. (2000) Polymerization, three-dimensional structure and mechanical properties of Dictyostelium versus rabbit muscle actin filaments. J. Mol. Biol. 303: 171-84.

Hoenger, A., Thormaehlen, M., Diaz-Avalos, R., Doerhoefer, M., Goldie, K., Mueller, J., and Mandelkow, E. (2000) A new Look at the Microtubule Binding Patterns of Dimeric Kinesins. J. Mol. Biol. 297, 1087-1103.
Mandelkow, E. and Hoenger, A. (1999) Structures of Kinesin and Kinesin-Microtubule Interactions. Curr. Opin. Cell. Biol. 11, 34-44.

Steinmetz, M. O., Hoenger, A., Tittmann, P., Fuchs, K., Gross, H., and Aebi, U. (1998) An Atomic Model of Actin Tubes: Combining Electron Microscopy and X-ray Crystallography. (1998) J. Mol. Biol. 278: 703-11.

Hoenger, A., Sack, S., Thormaehlen, M., Marx, A., Mueller, J., Gross, H., and Mandelkow, E. (1998) Image Reconstructions of Microtubules Decorated with Monomeric and Dimeric Kinesins: Comparison with X-ray Structure and Implications for Motility. J. Cell Biol. 141, 419-430.

Steinmetz, M. O., Stoffler, D., Hoenger, A., Bremer, A., and Aebi, U. Actin: from Cell Biology to Atomic Detail. (1997) J. Struct. Biol. 119, 295-320.

Sosa, H., Dias, P. D., Hoenger, A., Whittaker, M., Wilson-Kubalek., E., Sablin, E., Fletterick, R., Vale, R. D., and Milligan, R. A. A Model for the Microtubule-Ncd Motor Protein Complex obtained by Cryo-Electron Microscopy and Image Analysis. (1997) Cell, 90, 217-224.

Sosa, H., Hoenger, A., and Milligan, R. A. (1997) Three Different Approaches to Motor decorated Microtubule 3-D structure. J. Struct. Biol. 118, 149-158.

Hoenger, A., and Milligan, R. A. (1997) Motor Domains of Ncd and Kinesin interact with Microtubule Protofilaments with the same Binding Geometry. J. Mol. Biol. J. Mol. Biol. 265, 553-564.

Hoenger, A., and Milligan, R. A. (1996) Polarity of 2-D and 3-D maps of Tubulin sheets and motor-decorated Tubulin Sheets. J. Mol. Biol. 263, 114-119.

Hoenger, A. and Aebi, U. (1996) 3-D Reconstruction from Ice-embedded and Negatively stained Biomacromolecular Assemblies: A Critical Comparison. J. Struct. Biol. 117, 99-116

Hoenger, A., Sablin, E. P., Vale R. D., Fletterick, R. J., and Milligan, R. A. (1995) Three dimensional Structure of a Tubulin-Motor Protein Complex. Nature 376, 271-274.