Hoenger Lab
University of Colorado at Boulder
Department of Molecular, Cellular and Developmental Biology

Structural and Functional Investigations
into Cytoskeletal Assemblies by
Cryo Electron Microscopy
and 3D Image Analysis

 

Publications

1. B. Chen, M. Doucleff, D.E. Wemmer, S. DeCarlo, H. Huang, E. Nogales, T.R. Hoover, E. Elena Kondrashkina, L. Guo, and B.T. Nixon. ATP ground- and transition states of the enhancer binding AAA+ ATPases support complex formation with their target protein, σ54. (2007) Structure, 15, 429-440.

2. Kostek S., Grob P., De Carlo S., Lipscomb S., Garczarek F., and E. Nogales (2006).
Molecular Architecture and Conformational Flexibility of human RNA Polymerase II.
Structure 14:1691-1700.

3. De Carlo S. Cryo-electron microscopy: plunge freezing. In: Handbook of Modern
Cryopreparation Methods for Electron Microscopy. Ed. CRC Press (2007). ISBN:
0849372275. In Press.

4. De Carlo S. Cryo-negative staining. In: Handbook of Modern Cryopreparation Methods
for Electron Microscopy. Ed. CRC Press (2007). ISBN: 0849372275. In Press.

5. De Carlo S., Chen B., Hoover T., Kondrashkina E., Nogales E., and B. T. Nixon (2006).
The structural basis of regulated assembly and function of the transcriptional activator
NtrC. Genes & Dev. 20: 1485-1495.

6. Jawhari A., Uhring M., De Carlo S., Crucifix C., Tocchini-Valentini G., Moras D.,
Schultz P. and Poterszman A. (2006). Structure and oligomeric state of human
transcription factor TFIIE. EMBO Rep. 7: 500-505.

7. Joniç S., Sorzano C. O. S., Thévenaz P., El-Bez C., De Carlo S. and Unser M. (2005).
Spline-based image-to-volume registration for three-dimensional electron microscopy.
Ultramicroscopy 103: 303-317.

8. Unser M., Sorzano C. O. S., Joniç S., El-Bez C., De Carlo S., Thévenaz P., Conway J.,
Steven A. C. and Trus B. L. (2005). Spectral Signal-to-Noise Ratio and Resolution
Assessment of 3-D Reconstructions. J. Struct. Biol. 149: 243-255.

9. De Carlo S., Fiaux H., and A Marca-Martinet C. (2004). Electron Cryo-Microscopy
Reveals the Mechanism of Action of Propranolol on Artificial Membranes. J. Lip. Res.
14: 61-76.

10. Spehner D., De Carlo S., Drillien R., Weiland F., Mildner K., Hanau D. and Rziha H.-J.
(2004). The appearance of the bona fide spiral tubule of Orf virus is dependent on an
intact viral 10 kDa protein. J. Virol. 78: 8085-8093.

11. Sorzano C. O. S., Joniç S., El-Bez C., Carazo J. M., De Carlo S., Thévenaz P. and Unser
M. (2004). A multiresolution approach to orientation assignment in 3-D electron
microscopy of single particles. J. Struct. Biol. 146: 381-392.

12. De Carlo S., Carles C., Riva M. and Schultz P. (2003). Cryo-negative staining reveals
conformational flexibility within yeast RNA Polymerase I. J. Mol. Biol. 329: 891-902.

13. Donnet M., Bowen P., Jongen N., Lemaître J., Hofmann H., Schreiner A., Jones A. G.,
Schenk R., Hofmann C. and De Carlo S. (2002). Successful scale-up from milliliter batch
optimization to a small scale continuous production using the segmented flow Tubular
reactor. Example of calcium carbonate Precipitation. In: Proceedings of the 15th
International Symposium on Industrial Crystallization; Sept. 15th-18th, Sorrento, Italy.
Associazione Italiana di Ingegneria Chimica, 2002: 1353-1358.

14. De Carlo S., El-Bez C., Alvarez-Rúa C., Borge J. and Dubochet J. (2002). Cryo-negative
staining reduces electron beam sensitivity of vitrified biological particles. J. Struct. Biol.
138: 216-226.

15. Bellon P. L., Cantele F., De Carlo S. and Lanzavecchia S. (2002). A trajectory-based
algorithm to determine and refine Euler angles of projections in three-dimensional
microscopy: improvements and tests. Ultramicroscopy 93: 111-121.

16. Braissant O., Perez A., De Carlo S., Morel M., Borruat G. and Roten C.-A. (2001).
CENTRIFUGE: A Microgravity Experiment realized by Lausanne University and Federal
Institute of Technology in Lausanne students during the European Space Agency (ESA)
Parabolic Flight Campaign. Bull. Soc. Vaud. Sc. Nat. 87: 203-209.

17. De Carlo S., Adrian M., Kälin P., Mayer J. M. and Dubochet J. (1999). Unexpected
property of trehalose as observed by cryo-electron microscopy. J. Microsc. 196: 40-45.

18. Roten C.-A., Galluser A., Borruat G., Udry S. D., Niederhäuser G., Croxatto A., Blanc
O., De Carlo S. and Karamata D. (1998). Interplanetary biological transfer of bacteria
entrapped in small meteorites: analysis of bacterial resistance to impact in ballistic
experiments. In: Proc. Mars Soc. Found. Conv. Edited by Robert Zubrin, published by
Univelt, California, USA.

University of Colorado, MCDB, 347 UCB, Boulder, CO 80309-0347